Course material for Esbatech course

15/12/2008

Session 1

Basic theory of curve fittting and dose interpolation. How to estimate precision of the dose estimate for an unknown sample. Dose response curves, 3PL, 4PL, 5PL and exponential.

Session 1 power point (you have a hard copy, but this version has some answers)

5pl.pdf A paper with lots of details about the 5PL model. Paul G. Gottschalk, John R. Dunn The five-parameter logistic: A characterization and comparison with the four-parameter logistic . Analytical Biochemistry 2005.

Session 2

Fitting an interpolating dose-response curves in Prism 4 and EXCEL, confidence intervals for the unknown estimates, precision profiles and a statistical approach to the LLQ.

Session 2 practical word document (you have a hard copy, but this version has some answers)

3pl-4pl-5pl.xls Dose-response functions. Change the parameters to see the effect on the curves.

4PL.pzf Prism 4 worksheet with data suitable for fitting a 4PL model.

4PL.xls Dose interpolation worksheet template for a 4PL model.

newexp.pzf Prism 4 worksheet with data suitable for fitting an exponential model.

exponential.xls Dose interpolation worksheet template for an exponential model.

Session 3

session3_with_answers.ppt Power point presentation. You have a copy but this ones has more details of how to fit models and is the one used in the lectures.

Weighting, parallelism and comparative assays.

Comparative_assays2.pzf Data for comparative assays of two agents as fitted at Esbatech.

Comparative_assays2_shared_parameters.pzf Data forcomparative assays of two agents with new equation that defines shared parameters..

Comparative_assays3_shared_parameters.pzf Data forcomparative assays of three agents fitted with shared parameters. This is fitted with 3 ED50s. Can you write it in terms of RPs.?

P4Global.pdf Global nonlinear regression with Prism 4. Harvey J. Motulsky (GraphPad Software) and Arthur Christopoulos. A paper explaining how to fit several curves together from the Graphpad web site

Session 4

session4.ppt Power point presentation. You have a copy.

replicates_and_tests_of_fit.xls Spread sheet for screening replicates abd testing fit.